{
  "inputs_sha256": "acffc16a75bfdbf2164db8641f816713c3a09d6cbd4250fe32837ab9fd3dc89f",
  "hash_convention": "UTF-8 text after universal-newline normalization to LF",
  "analysis_status": "Descriptive source reanalysis, no new significance/equivalence tests or reproductive-risk model",
  "PC3_cell_selectivity": {
    "rapamycin": {
      "C1_ic50_pM": {
        "n": 4,
        "mean": 63.30875,
        "sample_sd": 18.539744161755127,
        "sem": 9.269872080877564
      },
      "C2_ic50_pM": {
        "n": 4,
        "mean": 534.8955,
        "sample_sd": 257.4247456772561,
        "sem": 128.71237283862806
      },
      "paired_replicate_C2_C1_selectivity": [
        11.138165864518875,
        16.67567071411712,
        1.8753832100804584,
        9.088419859381116
      ],
      "mean_of_paired_ratios": {
        "n": 4,
        "mean": 9.694409912024392,
        "sample_sd": 6.119009618852125,
        "sem": 3.0595048094260626
      },
      "ratio_of_mean_ic50s": 8.448997966315872
    },
    "DL001": {
      "C1_ic50_pM": {
        "n": 4,
        "mean": 74.93599999999999,
        "sample_sd": 24.883770118961746,
        "sem": 12.441885059480873
      },
      "C2_ic50_pM": {
        "n": 4,
        "mean": 26245.41275,
        "sample_sd": 14227.642744789488,
        "sem": 7113.821372394744
      },
      "paired_replicate_C2_C1_selectivity": [
        829.2318040543676,
        587.6838400281579,
        94.54376231254774,
        222.04815010511405
      ],
      "mean_of_paired_ratios": {
        "n": 4,
        "mean": 433.37688912504683,
        "sample_sd": 336.6400018476735,
        "sem": 168.32000092383674
      },
      "ratio_of_mean_ic50s": 350.2377061759368
    },
    "relative_DL001_vs_rapamycin": {
      "ratio_of_compound_mean_paired_selectivities": 44.70379250082162,
      "ratio_of_compound_ratios_of_mean_ic50s": 41.45316492822587,
      "cross_compound_replicate_pairing_assumed": false,
      "new_p_value": null,
      "interpretation": "Different descriptive estimands, not conflicting experiments. No reproductive or tissue selectivity follows from PC3 ratios."
    }
  },
  "tissue_pathway_series": 18,
  "tissue_pathway_interpretation": "Different tissues and source sex/context; zeros retained as normalized readouts, not proof of absent kinase activity. No parent tissue exposure is measured by these values.",
  "reported_whole_blood_compound_concentration_ng_per_mL": {
    "DL001": {
      "n": 9,
      "mean": 186.27777777777777,
      "sample_sd": 34.14135241088795,
      "sem": 11.380450803629316
    },
    "rapamycin": {
      "n": 18,
      "mean": 257.2055555555556,
      "sample_sd": 174.50345512245082,
      "sem": 41.13085881918912
    }
  },
  "whole_blood_DL001_to_rapamycin_mean_ratio": 0.7242369915977276,
  "blood_parent_exposure_qualification": "Single reported post-administration whole-blood time point, not AUC, oral F, unbound or testis concentration. Assay validation/specificity not established from the retrieved main-text methods. Cannot match to female Figure3d animals or infer cause of pathway selectivity.",
  "adult_mouse_testis_mass": {
    "rapamycin_to_control_reported_mean_ratio": 0.4609740670461733,
    "reported_mean_difference_mg_per_pair": -85.22,
    "control_n_animals": 5,
    "rapamycin_n_animals": 4,
    "individual_observations_or_new_confidence_interval": null,
    "human_testicular_volume_inference": null,
    "mechanistic_attribution_to_C2_alone": null
  },
  "human_transplant_descriptive_contrasts": {
    "total_sperm_count_mean_difference_million": -263.59999999999997,
    "motile_sperm_mean_difference_percentage_points": -18.8,
    "n_semen_by_regimen": null,
    "adjusted_causal_effect": null,
    "probability_of_infertility_or_recovery": null,
    "comparison_to_DL001": null,
    "interpretation": "Unadjusted differences of reported abstract means in transplant recipients. Respondents are not the established semen sample size; no healthy-person effect or testicular-volume estimate follows."
  },
  "candidate_joint_evidence_gates": {
    "DL001": {
      "joint_human_reproductive_protection_comparison_eligible": false,
      "missing": [
        "testis_cell_native_parent_exposure",
        "testis_cell_C1_C2_time_context",
        "direct_reproductive_endpoints",
        "matched_specified_intended_function",
        "same_relevant_population_for_joint_comparison",
        "human_reproductive_candidate_comparison"
      ],
      "human_reproductive_risk_reduction_estimate": null
    },
    "rapamycin": {
      "joint_human_reproductive_protection_comparison_eligible": false,
      "missing": [
        "testis_cell_native_parent_exposure",
        "testis_cell_C1_C2_time_context",
        "matched_specified_intended_function",
        "same_relevant_population_for_joint_comparison",
        "human_reproductive_candidate_comparison"
      ],
      "human_reproductive_risk_reduction_estimate": null
    }
  },
  "human_safe_analogue_or_equivalent_benefit_identified": false,
  "recovery_qualification": {
    "source_location": "Figure 7 and results/abstract",
    "n_animals_per_group": 3,
    "followup_months": 2,
    "reported_outcomes": "testis mass and motility described as restored; sperm counts increased; abstract qualifies the effect as partially reversible",
    "raw_individual_count_or_motility_values": null,
    "numeric_recovery_probability": null,
    "human_recovery_guarantee": false,
    "qualification": "Do not reinterpret non-significant testis mass as demonstrated equivalence or recovery of every reproductive outcome. No figure digitization is substituted for raw data."
  }
}